Bioassay Technical Support

Bioassay Lab photoThe Baroid Bioassay Laboratory Group in Houston performs chronic and acute aquatic toxicity tests on water-, oil-, and synthetic-based drilling fluids, stock base fluids, products, brines, produced waters, and effluent discharges. Oil and grease tests are performed on produced waters and brines. Sheen tests are performed on drilling fluids. Biodegradation tests are performed on existing products and research product candidates for Gulf of Mexico or North Sea applications

Aquatic organisms that are cultured and/or tested in the lab include the following:

  • Mysid shrimp - used primarily to determine toxicity of drilling fluids and products for discharge on cuttings in the Gulf of Mexico
  • Leptocheirus amphipod - used to determine toxicity of base synthetic fluids, synthetic drilling fluids and products for discharge on cuttings in the Gulf of Mexico
  • Sheepshead minnow - used to determine toxicity of drilling fluids and products discharged to seawater
  • Skeletonema algae - used to determine toxicity of drilling fluids and products discharged to seawater
  • Daphnia/Ceriodaphnia - used to determine toxicity of effluent discharge to freshwater

The specific bioassay, biodegradation, and oil and grease tests performed by the lab include the following:

  • 96-hr definitive acute toxicity tests on any of the cultured species.
  • 48-hr rangefinder acute toxicity tests on any of the cultured species.
  • 48-hr definitive acute toxicity tests on any of the cultured species.
  • 96-hr leptocheirus sediment toxicity tests on drilling fluids.
  • 10-day leptocheirus sediment toxicity tests on base synthetic fluids.
  • EPA 1664 oil and grease tests on produced fluids or brines.
  • OECD 306 and BODIS seawater aerobic biodegradation tests on drilling fluid product and components.

Specific EPA, ASTM, COE, and OECD test protocols are followed to meet state and federal discharge monitoring regulations. The Baroid Bioassay Laboratory's Quality Assurance Program guarantees that the accuracy and precision of reported results from the lab have been adequately monitored and exceed minimum reliability requirements for the appropriate protocols. The Program meets NELAC and ISO9001 certification criteria. The lab is also Good Laboratory Practices (GLP) qualified to submit test results acceptable to North Sea regulatory agencies.

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